Cytotoxicity of Mg-1.6Gd Alloys After Hot Rolling at an 80% Reduction Level as Implant Material

EasyChair Preprint no. 10807

Abstract

The high number of traffic accidents in Indonesia causes many victims which suffer from broken bones. Treatment which can be conducted is bone implants installation. Furthermore, magnesium is an attractive material for biodegradable bone implants since its physical properties are almost similar to human bone, but in the human body, magnesium experiences rapid dissolution before new tissue grows. Therefore, it needs to be combined with gadolonium in order to delay the solubility of magnesium. Before the Mg-Gd alloy was used as implant material, it is important to conduct a cytotoxicity test by using osteoblast cells. In order to obtain cell viability after it was given treatment, this study used the MTT Assay method. Furthermore, Mg-1,6Gd samples which had rolled with 80% at various temperatures of 400 ^⃘C, 450 ^⃘C, 500 ^⃘C, and 550 ^⃘C were inserted into plates which contained osteoblast cells. After the samples were incubated for 3, 7, and 14 days, the MTT was dissolved into the plate and it was read with Elisa Plate Reader. The study shows that the sample which has rolled with 80% reduction is non- toxic since the average viability value is above 70%. In addition, samples which had rolled with 80% at a temperature of 400  ^⃘C have stable viability value which is above 70%.

Keyphrases: cytotoxicity, Gadolinium, Implant Bone, Magnesium, Osteoblast Cells

@Booklet{EasyChair:10807,
  author = {Oknovia Susanti and Hameri Annisa},
  title = {Cytotoxicity of Mg-1.6Gd Alloys After Hot Rolling at an 80% Reduction Level as Implant Material},
  howpublished = {EasyChair Preprint no. 10807},

  year = {EasyChair, 2023}}